Wells B446 Manual do Utilizador descarregar pdf (Página 4)

Pharmacology+&+Toxicology+Core+Facilities+ + SOP+#:15928.B446.001++

+ + Version+#:++

Author:+J.+Thompson+&+T.++Szasz+ Page%%%%%%of%%8+ Approval+Date:+

+ + +

3.5 <(=?)-O%>$=6(# $6%%

a. &=7A% W6$0% Each% time% the% Bio‐Tek% µQuant% Microplate%

Spectrophotometer%is%used,%clean%all%exposed%surfaces%with%a%cloth%

dampened% with% distilled% water% or% distilled% water% and% a% mild%

detergent.%

b. &U$#O%@)X%>",-A60%Inspect%cables%and%power%cords%to%ensure%their%

integrity.%%

3.6 &X=+* ?$% *#"7$S(#$% L"#% T"M#O% *#"-$),% =6 6=O%

6*$7-#"*A"-"+$-#)7% +$=6( #$+$,-Y% Bio$Tek,)

Quant)

Microplate)Spectrophotometer)Use)%

a. Turn%on%the%computer%from%the%ON%button.%

b. Turn% on% µQuant% Spectrophotometer% from% the% ON/OFF% switch%

on%the%right%side.%

c. Double%click%KC%Junior%software%on%the%Desktop%and%click% OK%on%

the%initialization%window.%

d. Click%on%Open%Protocol%and%select%the%saved%protocol%from%the%

drop‐down% list.% In% this% case% it% will% be% “JLG% Lowry”.% To% create%

New%Protocol%for%a%different%assay,%refer%to%the%user’s%manual,%

keeping% in% mind% the% assay% specifics% (such% as% appropriate%

absorbance%wavelength).%

e. Click% on% Modify% Protocol.% A% window% will% open% with% the%

following%tabs:%General,%Read%Method,%Template,%Curve,%Cutoff,%

Reports.% If% the% saved% protocol% fits% the% intended% use% of% the%

experiment,% the% only% tab% requiring% modification% from% default%

settings% will% be% “Template”.% On% the% “Template”% tab,% select% the%

appropriate% Well% Type% from% the% drop‐down% list% to% match% the%

actual%well%content:%“Blank”,%“Standard”%or%“Sample”.%To%insert%

selections,%click%and%drag%over%the%96‐well%template%area,%with%

sample% numbers% automatically% increasing% in% a% left‐right% and%

up‐down% direction.% If% replicates% of% the% same% sample% number%

are% included,% change% the% replicate% number% and% indicate% their%

direction% (e.g.% down% and% across).% To% delete% selections,% select%

“Clear”%from%the% Well% Type%drop‐down% list%and% click%and% drag%

over%the%wells%that%need%to%be%deleted.%

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Wells B446 Manual do Utilizador Página 4